Cloning and analysis of Trp1 and Trp2 genes from white hair black eyes rabbit iris
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    Abstract:

    Objective To isolate and obtain the complete sequence of Trp1 and Trp2 genes from white hair black eyes(WHBE) rabbit iris, which represent the second and third members of the tyrosinase-related gene family, and to identify the sequences of protein TRP1 and TRP2.MethodsThe WHBE rabbit iris whole mRNA was reversely transcripted to cDNA. The cDNA was isolated from mouse, rat and human using homologous primers. The fragments and complete sequences of the cDNA were obtained by PCR and RACE. ResultsThe WHBE rabbit coding region was sequenced completely to yield 1604 bp full length and shared 87.9% identity with the human sequence and 82.7% identity with the mouse sequence. The Trp1 mature protein sequence had 513 amino acids and shared 89.8% identity with the human sequence and 86.6% identity with the mouse sequence. The WHBE rabbit Trp2 coding region was sequenced completely to yield 1554 bp full length and showed 83.2% identity with the human sequence and 81.9% identity with the mouse sequence. The WHBE rabbit Trp2 mature protein sequence had 494 amino acids and shared 84.2% identity with the human sequence and 84.4% identity with the mouse sequence. ConclusionsThe protein sequences of TRP1and TRP2 obtained from WHBE rabbit iris in this study show a high homology to TYR and show structural characteristics of the tyrosinase-related protein family. 

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