Construction and identification of DNA libraries enriched for microsatellite repeat sequences of Chinese hamster
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Q95-33

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    Abstract:

    Objective To screen the microsatellite loci of Chinese hamster DNA to serve the genetic studies of germplasm resources,classification and evolution of Chinese hamsters.Methods Genomic DNAs from Chinese hamster was fragmented by ultrasonication.The fragments in size from 500 bp to 1000 bp were recovered by 2% agarose gel electrophoresis and ligated to SNX linkers with T4 DNA ligase,then denatured and hybridized to 14 biotinylated oligonucleotides.The biotinylated hybrids were retained on magnetic beads accor...

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