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| Construction of a 5-Lipoxygenase Transgenic Mice |
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| DOI： |
| KeyWord:5-lipoxygenase;Atheroselerosis;Transgenic mice |
| ZHANG Mei-ying,WU Hong-lian,YANG Wei,LI Zhao-yang,DONG Wan-wei,ZHOU Sheng-lai,YU Yang,WANG Wei,Lü Xiang-chuan,QIN Ying,ZHENG Zhi-hong,WANG Lu-zeng |
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| Abstract: |
| Objective To construct a 5-lipoxygenase(5-LO) transgenic mouse model of atherosclerosis.Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice.PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues.RT-PCR and Western blot analysis were used to detect the gene transcription and expression.Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.Expression ... |
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