Expression of Nerve Growth Factor (NGF) in Mouse Testes at Different Developmental Stages
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KeyWord:Nerve growth factor(NGF);Testis,mouse;Real-time PCR;Immunohistochemistry
HU Yong-ting,CHEN Wen-hua,ZHANG Ying,NIE Xiang-ting
HU Yong-ting~1,CHEN Wen-hua~2,ZHANG Ying~1,NIE Xiang-ting~1(1.College of Veterinary Medicine , Animal Science,Shanxi Agricultural Univercity,Taigu 030801,China,2.The Center for Disease Control of Yuncheng City,Yuncheng 044000,China)
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      Objective To study the location and the expression of nerve growth factor(NGF) in mouse testes at different developmental stages.Methods The testes were taken out from mice at different developmental stages,respectively.Parts of the testis tissue were used to extract total RNA and real-time PCR was used to detect the expression of NGF.The rest tissues were fixed and embedded in paraffin.SABC immunohistochemical staining was used to observe the location of NGF in the testes. Results Real-time PCR results sho...
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