Objective To establish an experimental animal model with insulin resistance and/or type 2 diabetes mellitus.Methods High energy fructose diet was given to mesocricetus to produce insulin resistance and hyperlipidemic models.Diabetes was induced by a single intraperitoneal injection of streptozotocin(30 mg/kg)on these insulin resistant animals.Normal animals were used as control.Serum glucose,lipid profiles and insulin levels were measured to verify the diabetic and hyperlipidemic models.The liver,pancreas and aortic arch were examined to distinguish the histological difference among the animals of three groups.Results The mesocricetus fed with high energy fructose diet for 6 weeks developed insulin resistance,obesity and dislipidemia,80% of which became type 2 diabetic after single intraperitoneal injection of streptozotocin.Conclusion Insulin resistant and diabetic mesocricetus models can be produced successfully with high energy fructose diet and single intraperitoneal injection of streptozotocin.