Establishment Of a Rat Model of Fetal Growth Restriction by Interruption Of the Uterine Artery B1ood Flow
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R-332

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    Abstract:

    Objective To establish an experimental animal model of fetal growth restriction(FGR) by temporary interruption of the uterine artery blood flow during late stage of pregnancy,and provide a useful animal model to investigate the FGR mechanism induced by hypoxia.Methods Based on the fact that the rat uterine artery is a branch of ovary artery,the pregnant rats undergone an operation temporarily interrupting the ovary artery blood flow at 15th day of pregnancy and caesarean birth at 21~(st) day of pregnancy.After the caesarean birth,the body weight of fetus,weight of placenta,weight of the brain,heart,liver,lungs and kidneys of the fetus in every group were compared.The prognosis of each group and the effect of different time of artery blood interruption(10 min,20 min,30 min and 40 min) were compared and analyzed.Results The rat FGR model was successfully established by interrupting the ovary artery in pregnant rats for 20 minutes.This method was simple to perform and needs less time than the operation interrupting the artery blood for 30 or 40 minutes,while the fetal mortality was similar between experimental group and control group (P>0.05).In the rat model,the weights of new-laid rats and their placenta,brain,heart,liver,lungs and kidneys were all decreased in comparison with those in control group(P<0.05).Conclusion By temporarily clamping the ovary artery blood flow in pregnant rats,an animal FGR model has been successfully established in rats.The model is simple to perform and well reproducible,with autogenous control,and provides a useful approach in FGR research.

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  • Received:
  • Revised:April 15,2006
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