Objective To isolate and identify Sendai virus strain in Heilongjiang province, and to be used as a diagnostic antigen in detecting SV serum antibody of laboratory animals. MethodsThe virus was isolated from conventional mice lungs through chicken embryo passages and identified by haemoglutination test, haemoglutination blocking test and constitution genes sequencing. The virus was purified by sugar density gradient centrifugation and was used as an immunogen to laboratory animals. The titers of immune serum were detected by standard kits. ResultsTwo virus strains with haemoglutination characteristics were isolated from 150 portions of mouse lungs, which were identified as Sendai virus through virus morphological characterization, serum detection and constitution gene sequencing. The nucleocapsid (N)gene sequencing result showed that the nucleotide sequence and deduced amino acid sequence of the isolated virus shares 99.6% and 99.0% homology with Fushimi strain, respectively. ConclusionSendai virus HLJ strain has been isolated and identified, which can be used to develop diagnostic kits for SV antibody detection.