Effects of HSP70 on the NOS Activity of Rat Liver after Ischemia Reperfusion
DOI:
Author:
Affiliation:

Clc Number:

R363

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Objective To investigate the protective effect of heat stress pretreatment on the hepatic ischemia resperfusion (I/R) injury and its mechanism of action. Methods To establish the rat model of hepatic ischemia resperfusion injury using Pringle's maneuver with or without heat stress pretreatment. The rats were randomly divided into pretreatment group (HP+IR) and non-pretreatment group (IR). The activity of NOS in the liver, the activity of serum enzyme LDH and the pathological changes of the liver were assessed at 0, 4, 8, 12 and 24 h after I/R. Results At each time point set after I/R, the activity of NOS in the liver of HP+IR group was lower than that of IR group. Maximal HSP70 expression was observed at 12 h after heat pretreatment. While in the HP+IR group, the level of liver enzyme was significantly reduced and the pathological changes were improved in comparison with those in IR group. Conclusion HSP70 induced by heat pretreatment protects the liver against I/R injury may be through inhibiting the NOS activity and therefore reducing the insults of oxygen free radicals on the liver.

    Reference
    Related
    Cited by
Get Citation
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:
  • Revised:January 21,2005
  • Adopted:
  • Online:
  • Published: