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| Construction of pcDNA3-hBMP2 Vector and Its Expression in Rabbit Bone Marrow Stromal Cells |
| Revised:December 22, 2004 |
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| DOI： |
| KeyWord:hBMP2 |
| CHEN Jun-jiang,LIU Dan-ping,CUI Da-xiang,WANG Wei,ZHANG Zheng,GUO Tao,ZHANG Nan |
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| Abstract: |
| Objective To construct human bone morphogenetic protein-2(hBMP-2 ) expressing eukaryotic vector(pcDNA3-hBMP2) and examine its expression in rabbit bone marrow stromal cells(MSCs).Methods pcDNA3-hBMP2 vector was constructed using gene clone and recombination technique.After being confirmed by enzyme cut,pcDNA3-hBMP2 was transfected into cultured rabbit MSCs in vitro with the help of liposome reagent FuGENE6.The expression of hBMP-2 was determined by in situ hybridization and immunohistochemical analysis.Results The pcDNA3-hBMP2 vector was constructed successfully.The rabbit MSCs transfected by pcDNA3-hBMP2 showed transient and stable expression of BMP2.Conclusion pcDNA3-hBMP2 can express hBMP2 in bone marrow stromal cells. |
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