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| Application of Ovary Transplantation in Pancreatic Cancer Transgenic Mice Propagation |
| Revised:December 06, 2004 |
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| DOI： |
| KeyWord:Mouse,Transgene,Ovary,Breeding,Serial Passage |
| LIU Ying,SUN Qiang,WANG Guang-feng,LIU Jin,ZHENG Yi-mei,DONG Juan,CAO Hui,LIU Quan-hai,LI Hou-da |
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| Abstract: |
| Objective To study the application of ovary transplantation for preserving pancreatic cancer transgenic mice.Methods Ovary of SV40T positive transgenic mice were transplanted into ovarectornized FVB female mice through micro-surgery,then were crossed with normal FVB male.The positive offsprings were confirmed by PCR.Ovary tissue slides of foster mother were examined.Results Ovaries of 19 donors were transplanted into 32 female mice,among which 21 were pregnant.96 in 159 offsprings survived.35 with a positive transgenic mice were identified by PCR,with a positive rate of 25.4%.Conclusion Ovary transplantation may be used for transgenic mice preservation. |
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