Studies on virological and serological detection of SARS Animal Model of Rhesus monkeys
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    Abstract:

    Successful development of anti-SARS drugs and vaccines depends greatly on a reliable and sensitive animal model. In April 2003, we firstly inoculated rats, mice, rabbits and guinea-pigs, but no animals were sensitive to SARS-CoV infection. Late in May, We tried to infect 3 kinds of primates, and then Rhesus monkey(Macaca mulata) and Cynomogus Macaques (Macaca fascicularis) were found to be sensitive. Here, we report that a SARS animal model has been successfully established by inoculating SARS coronavirus into Rhesus monkeys through the nasal cavity and bronchi. We have found that although all the 8 animals show light evident clinical signs except for a transient fever 2 to 3 days after inoculation of SARS SARS-CoV, the SARS virus RNA was detected for 10-15 days using nested RT-PCR in pharyngeal swab samples in 8 cases 5 days following inoculation, the SARS coronavirus-specific IgG was detcetbale in the serum of monkeys 11-15 days after inoculation. In addition, necropsy findings of lung tissues showed typical SARS changs. Taken together, the pathological changes, immune response and virus excretion in this SARS animal model may provide insight into the mechanism of SARS infection and may greatly facilitate the screening of anti-SARS drug and vaccine evaluation.

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