Moleculor Cloning and Characterization of SLA-DQ cDNA of the Banna Mini-Pig Inbred Line Family 133
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Q78

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    Abstract:

    Objective Banna Mini-pig Inbred Line 133 family(BMI-133) is one of the sub-lines which is differentiated from the Banna Mini-pig Inbred Line(BMI) with stable genetics and high homozygosity. It has been found that BMI-133 has many similarities with human such as organ anatomy, physiology and biochemcal indexes and so on. The purpose of this study is to explore the potential application of BMI-133 in xeontransplantion for treatment of human diseases. Method The DQ genes of BMI-133 were cloned and sequenced by RNA extraction, reverse transcription, cDNA amplification and transformation. Two complete open reading frames (ORF) have been obtained. Result The cDNA of DQA BMI-133 has 830 nucleotides and that of DQB has 1103 nucleotides. They encode 255 and 262 amino acids, respectively. Conclusion The results showed that BMI-133 has distinct differences with other mini-pigs reported, they are two new alleles. They also have high similarity to the counterpart sequences of human. It is expected that following further research on BMI-133, it may become a most suitable animal for xeontransplatation.

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  • Received:
  • Revised:July 09,2004
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