Objective In order to set up an accurate and convenient method for genetic assessment of macaca rhesus. Method DNA polymorphic microsatellites of macaca rhesus were analyzed using PCR technique in 20 macaca rhesuses.Res ults\ Nine polymorphic microsatellites loci, 4 non-polymorphic microsatel lites loci, a nd alleles of 2 microsatellites loci in a smaller number were screened. Conclusion\ An efficient, accurate and convenient PCR method charac terizing DNA polymorphic satellites loci had been established which can be used for genetic assessment of macaca rhesus.