Objective To analyze whether there are sensitive PRNP genes in the r hesuses used in spaceship analog experiments, and to provide a reference for ass essment of pathological brain alterations of these rhesuses tprovoked by exposur e to hypergravity.Method\ Total DNA was isolated from peripheral bl ood ymphocyte s of 17 healthy male adult rhesuses, which would be used in corresponding parabo lic flight experiments. The PRNP gene was amplified by PCR and analyzed.R esults\ The cloned PRNP gene is 762 nucleotides long and contains the en tire PRNP coding sequence, which has no intron and has more than 95% homology w ith the published gene sequence, deducing 253 amino acids and molecular weight ( MW) of 27.6 kd.Conclusion\ There are no sensitive PRNP genes in th e rhesuses, which provide a reference for judgement of pathological brain alterations of these rhesuses as e xposed to hypergravity.