Homology and Allele Analysis of the NS Gene of Avian Influenza Virus Isolates
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S852.65

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    Abstract:

    Objective To clone and sequence the non-structure (NS) protein gene of avian influenza virus (AIV) isolates and analyze the homology and allele of the NS gene, and to set up a method for detection of antibodies against NS prote in. Method The NS gene of 7 AIV isolates was amplified by reve arse transcription polymerase chain reaction (RT-PCR). The DNA fragments were c loned into pGEM-T vector and sequenced subsequently. The nucleotide sequence wa s compared with GenBank data and the phylogenetic tree of NS gene was generated. Results The entire ORF sequences of NS1 and NS2 protein were obtained. Homology analysis showed that the NS gene of these 3 H9N2 strains exhi bited 96% ~ 98% identity with each other, as to 2 H5N1 strains, 91.6%, while the 2 H7N2 strains showed 98.9% ident ity. The identity between H5N1 and H9N2 isolates was more than 90%, and about 60 % ~ 70% between H7N2 and the other two subtypes. In NS gene phylogenetic tree, t he H5N1 and H9N2 isolates were situated in branch of allele A, while H7N2 isolat es in branch of allele B. The location of the 3 H9N2 isolates was very close, ha ving the same origin with some of the H5N1 isolated in Hong Kong and Guangdong. Conclusion There is a significant defference in the nucleotide sequence identity of NS gene of AIV isolated in China. The NS gene of these sev en AIV isolates belongs to different alleles, allele A and allele B.

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  • Received:
  • Revised:October 09,2004
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