Objective To discuss the effect of in vitro fertilization (IVF), embryos and sperm cryopreservation in various mouse strains. Methods IVF was performed in 13 mouse strains(C 57 BL/6J,BALB/c,C3H/HeJ,ICR,KM,FVB,MRL,NOD,CBA,DBA/2,CD-1,BDF1,B6C3F1) in two laboratories of CARD and SLAC to compare the IVF, embryos thawed and embryos transfer. Results The fertility rates of fresh spermatozoa by in vitro were from 15.1% to 87.9%, and frozen sperm were about 8%-80%, the thawed rates of frozen embryos were from 42.6% to 83.9%, and 17.8% to 51.8% offspring were procreated after morphological normal 2-cell embryos were transferred to the oviducts of pseudopregnant recipient in all strains. Conclusion These results suggested that there were distinct differences in the rate of fertilization, thawed and transfer in different genetic background. But no significant differences(P>0.05) were found in each strain in CARD versus SLAC in spite of the high fertility rates(P<0.01) of frozen sperm in the former.