ObjectiveTo analyze the genetic purity of Chinese hamsters E Genealogy inbred strain. Method31 RAPD primers were selected and used to amplify 12 sam ples of E genealogy of Chinese hamster. The amplified fragment bands were analy zed to calculate the similarity index.Result In this study ,the similarity index of all samples ranged from 94 3 1% to 99 78%, averaging at 97 49%. ConclusionChinese hamster of Shanyi colony E genealogy has reached a high degree of genetic purity.