ObjectiveTo find out the influences of ages and sexes on normal blood biochemical parameters in the Lagurus lagurus closed colony. Methods 23 blood biochemical parameters of male group, female group, 1-2 monthes group and 24 monthes group in Lagurus lagurus closed colony were determined by using AEROSET auto blood biochemical analyser. Results The same parameters in two age groups had no difference, so did in two sex groups. Conclusion In this experiment, ages(1-2 monthes and 24 monthes) and sexes showed no influences on the 23 normal blood biochemical parameters in the Lagurus lagurus closed colony.