Objective To detect and analyse hematologic,serum biochemical and hemorheologic values in rhesus monkeys in anesthesia and unanesthesia.Methods Blood samples were obstained from 48 healthy rhesus monkeys in unanesthesia,30 blood samples obstained in anesthesia.All the samples were tested with automatic blood cellular analyzer,automatic blood biochemical analyzer and N6A automatic washing viscosimeter.Results In the anesthesia compared with unanesthesia,the values of GGT,AST,CREAT,TBIL,DBIL,P 3 ,TF in serum biochemistry,RBC,Hct,MCV,MPV,RDW,NEUTRO in hematology and plasma viscosity were significantly lower ( P <0.01),the values of ALT,ALP,LDH,HBDH were lower( P <0.05),on the contrary values of UA,Ca 2 ,CK MB,ALB,MCH,MCHC,PLT,LYM and MEDIAN were higher( P <0.01).Conclusion These reference values were affected in rhesus monkeys anesthetized.