Objective To collect endometrium with complete structure and normal function Methods Three means of cutting,scraping and squeezing were comparatively studied Results The endometrium by squeezing was in the shape of cylinder with integrated tissue structure including endometrial epithelium,gland and capillary Moreover the endometrium situated in the mesometrium was incomplete Conclusion Squeezing the cornua uteri is the practicable way to collect the mouse endometrium