Abstract:seudomonas aeruginosa was detected from CV rats, CL mice and animalcaretakers, All the isolated strains were serotyped by using of diagnosticantisera.The results indicated that 33-7% of investigated animaes werecontaminited by Pseudomonas aeruginosa, and the same serotypos of P.aeruginosa were detected from animal caretakers oral cavities and fingersbefore and after they contact animals .According to the serotype results,Pseudomonas aeruginosa can be spreaded batween animal caretakers andtheir bred animals in one-way or two-way style.