首页期刊介绍编委会投稿指南期刊订阅广告合作留言板联系我们English
韩雨,陈晨,王鑫峰,张海阳,陈俞,许德玮,姚铁翼,邵晓云,徐绍业.雌性大鼠吸烟对仔鼠行为学影响的研究[J].中国实验动物学报,2022,30(6):810~818.
雌性大鼠吸烟对仔鼠行为学影响的研究
Effect of smoking on behavior of female rats
投稿时间:2022-03-18  
DOI:10. 3969 / j.issn.1005-4847. 2022. 06. 010
中文关键词:  香烟烟雾暴露  子代大鼠  神经系统  行为学  CRMP1  DAPK1
英文关键词:cigarette smoke exposure  offspring rats  nervous system  animal behavior  CRMP1  DAPK1
基金项目:
作者单位
韩雨 桂林医学院基础医学院,广西 桂林 541004 
陈晨 桂林医学院基础医学院,广西 桂林 541004 
王鑫峰 桂林医学院基础医学院,广西 桂林 541004 
张海阳 桂林医学院临床医学院,广西 桂林 541004 
陈俞 桂林医学院临床医学院,广西 桂林 541004 
许德玮 桂林医学院临床医学院,广西 桂林 541004 
姚铁翼 桂林医学院临床医学院,广西 桂林 541004 
邵晓云 1. 桂林医学院基础医学院,广西 桂林 541004
3. 桂林医学院脑与认知神经 重点实验室,广西 桂林 541004 
徐绍业 桂林医学院科学实验中心,广西 桂林 541004 
Author NameAffiliation
HAN Yu Basic Medicine College, Guilin Medical University, Guilin 541004, China 
CHEN Chen Basic Medicine College, Guilin Medical University, Guilin 541004, China 
WANG Xinfeng Basic Medicine College, Guilin Medical University, Guilin 541004, China 
ZHANG Haiyang Department of Clinical Medicine, Guilin Medical University, Guilin 541004 
CHEN Yu Department of Clinical Medicine, Guilin Medical University, Guilin 541004 
XU Dewei Department of Clinical Medicine, Guilin Medical University, Guilin 541004 
YAO Tieyi Department of Clinical Medicine, Guilin Medical University, Guilin 541004 
SHAO Xiaoyun 1. Basic Medicine College, Guilin Medical University, Guilin 541004, China.3. Key Laboratory of Brain and Cognitive Neuroscience, Guilin Medical College, Guilin 541004. 
XU Shaoye Science Experiment Center, Guilin Medical College, Guilin 541004 
摘要点击次数: 110
全文下载次数: 24
中文摘要:
       目的 探讨亲代成年雌鼠香烟烟雾暴露对子代大鼠行为学及大脑皮层坍塌反应调节蛋白 1 (CRMP1)、死亡相关蛋白激酶 1(DAPK1)蛋白表达的影响。 方法 将 18 只 8 周龄健康 SD 雌性大鼠随机分为烟熏 组(9 只)和对照组(9 只),烟熏组行香烟烟雾暴露构建烟熏模型,对照组正常饲养,烟熏 1 个月后将其与正常雄鼠合笼并继续烟熏直至分娩。 将烟熏组和对照组分娩后的子代大鼠( F1 代) 分别进行旷场实验、转棒疲劳实验、 Morris 水迷宫实验等动物行为学检测,分析亲代雌鼠香烟烟雾暴露后对其子代的焦虑情况、运动耐受能力、以及空间学习记忆能力的影响。 Western Blot 检测不同发育阶段( 0、7、21 d 和 4 周) 各组子代大鼠的大脑皮层组织中 DAPK1 和 CRMP1 蛋白表达情况。 结果 在旷场实验结果显示,烟熏组 F1 代子代大鼠在总区域中的运动总距离和平均运动速度明显增加(P< 0. 05),其中进入中央区的次数、在中央区的运动距离和停留时间均较对照组 F1 代 明显增加(P< 0. 05);转棒疲劳实验结果发现,烟熏组 F1 代持续运动时间以及运动距离较对照组均显著减少(P< 0. 05);Morris 水迷宫结果显示,烟熏组和对照组 F1 代的逃避潜伏期和到达平台的游泳路程无明显差异,但游泳速 度较对照组明显下降(P< 0. 05);烟熏组 F1 代在目标象限停留时间占比和进入目标象限次数均较对照组明显减少(P< 0. 05),且平均游泳速度和总游泳路程也明显下降(P< 0. 01);此外,Western Blot 结果表明,烟熏组 F1 代大 鼠脑组织中 DAPK1 和 CRMP1 在发育早期(0、7、21 d)的蛋白表达均较对照组下降。 结论 亲代雌鼠香烟烟雾暴露可能会增加其子代大鼠的焦虑情绪,降低运动耐受能力,并使得其空间记忆能力减退,这可能与子代大鼠脑组织中 CRMP1 和 DAPK1 分子的调控有关。
英文摘要:
       Objective To investigate the effects of passive smoking in adult female rats on behavior and expression of collapsin response regulatory protein 1 (CRMP1) and death-related protein kinase 1 (DAPK1) in the cerebral cortex of offspring. Methods Eighteen 8-week-old healthy female SD rats were randomly divided into the passive smoking group (n= 9) and control group (n= 9). The passive smoking group was treated with smoke to establish the passive smoking model, whereas the control group was treated normally. After 1 month of smoking, female rats were mated with males and continued smoking until delivery. Offspring (F1 generation) of passive smoking and control groups were evaluated by an open field test, rotary rod fatigue test, and Morris water maze test. These animal behavior tests were conducted to analyze the effects of passive smoking on anxiety, exercise tolerance, and spatial learning and memory abilities of the offspring. Moreover, CRMP1 and DAPK1 protein expression in cerebral cortex tissues of offspring was detected by Western Blot at various developmental stages (0, 7, and 21 days and 4 weeks ( adult)) after birth. Results The open field test showed that the total movement distance and average movement speed of the F1 generation in the passive smoking group were significantly increased in the total area ( P< 0. 05), and the number of entering the central area, and the movement distance and residence time in the central area were significantly increased compared with those of the control (P< 0. 05). The rotary rod fatigue test showed that the sustained movement time and movement distance of the F1 generation in the passive smoking group were significantly reduced compared with those in the control group (P< 0. 001). The Morris water maze showed that escape latency of the F1 generation had no significant difference between passive smoking group and control groups, but swimming speed was significantly decreased in the passive smoking group (P< 0. 001). Compared with the control group, the proportion of time and time of the F1 generation entering the target quadrant in the passive smoking group were significantly decreased (P< 0. 05), and the swimming speed and total swimming distance were also decreased significantly (P< 0. 01). Additionally, Western Blot showed that CRMP1 and DAPK1 protein expression in offspring of the passive smoking group was significantly lower than that of the control group at 0, 7, and 21 days. Conclusions Passive smoking in parental female rats promoted anxiety, reduced exercise tolerance, and impaired spatial memory of offspring, which may be related to regulation of CRMP1 and DAPK1 expression in the brain tissue of offspring.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 3270022 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会    中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb2@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码