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武振帅,纪鹏,魏彦明,徐誉彰,张晓松,武凡琳,任建明,李琛琛,华永丽,姚万玲,袁子文.黄芩-连翘配伍对脂多糖诱导小鼠急性肺炎模型的保护作用[J].中国实验动物学报,2022,30(6):800~809.
黄芩-连翘配伍对脂多糖诱导小鼠急性肺炎模型的保护作用
Protective effect of Scutellariae Radix-Forsythiae fructus in an acute pneumonia mouse model induced by lipopolysaccharide
投稿时间:2022-01-11  
DOI:10. 3969 / j.issn.1005-4847. 2022. 06. 009
中文关键词:  急性肺炎  脂多糖  黄芩  连翘  配伍
英文关键词:acute pneumonia  lipopolysaccharide  Scutellariae Radix  Forsythiae Fructus  compatibility
基金项目:
作者单位
武振帅 甘肃农业大学动物医学院,兰州 730070 
纪鹏 甘肃农业大学动物医学院,兰州 730070 
魏彦明 甘肃农业大学动物医学院,兰州 730070 
徐誉彰 甘肃农业大学动物医学院,兰州 730070 
张晓松 甘肃农业大学动物医学院,兰州 730070 
武凡琳 甘肃农业大学动物医学院,兰州 730070 
任建明 甘肃农业大学动物医学院,兰州 730070 
李琛琛 甘肃农业大学动物医学院,兰州 730070 
华永丽 甘肃农业大学动物医学院,兰州 730070 
姚万玲 甘肃农业大学动物医学院,兰州 730070 
袁子文 甘肃农业大学动物医学院,兰州 730070 
Author NameAffiliation
WU Zhenshuai College of Veterinary Medicine of Gansu Agricultural University, Lanzhou 730070, China 
JI Peng College of Veterinary Medicine of Gansu Agricultural University, Lanzhou 730070, China 
WEI Yanming College of Veterinary Medicine of Gansu Agricultural University, Lanzhou 730070, China 
XU Yuzhang College of Veterinary Medicine of Gansu Agricultural University, Lanzhou 730070, China 
ZHANG Xiaosong College of Veterinary Medicine of Gansu Agricultural University, Lanzhou 730070, China 
WU Fanlin College of Veterinary Medicine of Gansu Agricultural University, Lanzhou 730070, China 
REN Jianming College of Veterinary Medicine of Gansu Agricultural University, Lanzhou 730070, China 
LI Chenchen College of Veterinary Medicine of Gansu Agricultural University, Lanzhou 730070, China 
HUA Yongli College of Veterinary Medicine of Gansu Agricultural University, Lanzhou 730070, China 
YAO Wanling College of Veterinary Medicine of Gansu Agricultural University, Lanzhou 730070, China 
YUAN Ziwen College of Veterinary Medicine of Gansu Agricultural University, Lanzhou 730070, China 
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中文摘要:
       目的 研究黄芩-连翘不同比例配伍对脂多糖(lipopolysaccharide,LPS)诱导小鼠急性肺炎模型的保护作用差异。 方法 首先采用加热回流法制备不同配伍比例黄芩-连翘组合物。 选取健康 KM 小鼠 144 只,随机分为 12 组(正常对照组、模型组、地塞米松阳性药物组、9 个黄芩-连翘不同比例配伍组),每组 12 只,连续灌服相应药物 7 d,正常对照组和模型组给予等量生理盐水;第 8 天,除正常对照组外,其余组小鼠均采用 LPS(10 mg / mL) 滴鼻法建立小鼠急性肺炎模型。 造模 6 h 后迅速采样。 通过小鼠临床体征、日均体重增长量、采食量、脏器指数、小鼠血清 TNF-α、IL-1β 和 IL-6 含量、血常规、肺组织形态学观察及肺损伤病理组织学评分,评估黄芩-连翘不同比例 配伍组对小鼠急性肺炎模型的保护作用差异。 结果 建模后各组小鼠存活率为 100%。 正常对照组小鼠临床体征正常,呼吸音正常,反应灵敏;模型组小鼠反应迟钝,蜷缩扎堆,听诊发现肺部有啰音。 不同给药组较模型组相比,整体情况较好,尤其黄芩-连翘配伍组(1 ∶ 1,2 ∶ 3,1 ∶ 2)活动较多,反应较灵敏。 小鼠日均体重增长量、日均采食 量、肾指数、肝指数、脾指数和胸腺指数差异不显著(P> 0. 05);与正常对照组比,模型组肺指数、血清中 TNF-α、IL- 1β 和 IL-6 含量、血常规指标、肺组织损伤病理组织学评分显著升高(P< 0. 01 或 P< 0. 05),小鼠肺出现大量炎性 细胞浸润,肺泡壁明显增厚、SFTPC 蛋白在肺组织肺泡与肺泡间隔区域表达量显著增加,大量阳性细胞表达 SFTPC 蛋白;与模型组相比,黄芩-连翘配伍组(3 ∶ 1,3 ∶ 2,2 ∶ 1,1 ∶ 3,3 ∶ 0,0 ∶ 3)的心指数、黄芩-连翘配伍组(1 ∶ 2)的肺指数、黄芩-连翘配伍组( 1 ∶ 1,2 ∶ 3,1 ∶ 2) 小鼠血清中 TNF-α、IL-1β 和 IL-6 含量、黄芩-连翘配伍组( 1 ∶ 1, 2 ∶ 3,1 ∶ 2)血常规指标含量、黄芩-连翘配伍组(3 ∶ 2,1 ∶ 1,2 ∶ 3,1 ∶ 2)肺组织损伤评分显著降低(P< 0. 01 或 P< 0. 05),4 个黄芩-连翘配伍组(3 ∶ 2,1 ∶ 1,2 ∶ 3,1 ∶ 2)小鼠肺病理现象明显减轻,SFTPC 蛋白在肺组织肺泡与 肺泡间隔区域表达量显著减少,阳性细胞的表达量较少。 结论 黄芩-连翘配伍(1 ∶ 1,2 ∶ 3,1 ∶ 2)对 LPS 诱导的急性肺炎具有明显保护作用。
英文摘要:
       Objective To study the protective effect of various proportions of Scutellariae Radix-Forsythiae fructus in an acute pneumonia mouse model induced by lipopolysaccharide (LPS). Methods The Scutellariae Radix-Forsythiae fructus composition with various compatibility ratios was prepared by the heating reflux method . In total, 144 healthy KM mice were randomly divided into 12 groups ( normal control, model, dexamethasone positive drug, and nine groups of Scutellariae Radix-Forsythiae fructus at various proportions) with 12 mice in each group. Normal control and model groups were administered the same amount of normal saline and the other groups were administered corresponding drugs for 7 continuous days. On day 8, except for the normal control group, mice in other groups were treated with LPS (10 mg / mL) by dripping a solution into the nasal cavity to establish the acute pneumonia model. Samples were collected quickly at 6 hours after modeling. The protective effects of various proportions of Scutellariae Radix-Forsythiae fructus in the acute pneumonia mouse model were evaluated by clinical signs, average daily weight gain, feed intake, the viscera index, serum TNF-α, IL-1β and IL-6 contents, blood routine index, lung histopathological observation, and lung injury histopathological score. Results After modeling, the survival rate of mice in each group was 100%. The normal control group had normal clinical signs, breath sounds, and a sensitive reaction. Mice in the model group were unresponsive and huddled together with rales in the lungs detected by auscultation. Compared with the model group, the overall situation of the various administration groups was better. In particular, the Scutellariae Radix-Forsythiae fructus combination groups (1 ∶ 1, 2 ∶ 3, 1 ∶ 2) showed more activity and sensitive reactions. No significant differences were observed in daily body weight increase, daily feed intake, or kidney, liver, spleen, and thymus indexes (P> 0. 05). Compared with the normal control group, the lung index, serum contents of TNF-α, IL-1β and IL-6, blood biochemical indexes, and histopathological score of lung tissue injury were significantly increased in the model group ( P< 0. 01 or P< 0. 05) . The alveolar wall had significantly thickened, SFTPC protein expression in alveolar and interalveolar regions of lung tissue was significantly increased, and a large number of cells expressed SFTPC protein. Compared with the model group, the heart index of the Scutellariae Radix-Forsythiae fructus combination groups (3 ∶ 1, 3 ∶ 2, 2 ∶ 1, 1 ∶ 3, 3 ∶ 0, 0 ∶ 3) , the lung index of the Scutellariae Radix-Forsythiae fructus combination group ( 1 ∶ 2) , and the serum TNF-α, IL-1β And IL-6 content, blood routine index content of the Scutellariae Radix-Forsythiae fructus combination groups (1 ∶ 1, 2 ∶ 3, 1 ∶ 2) , lung tissue injury scores of the Scutellariae Radix-Forsythiae fructus combination groups ( 3 ∶ 2, 1 ∶ 1, 2 ∶ 3, 1 ∶ 2) were significantly decreased (P < 0. 01 or P < 0. 05) . The lung pathology of mice in the four Scutellariae Radix-Forsythiae fructus groups (3 ∶ 2, 1 ∶ 1, 2 ∶ 3, 1 ∶ 2) was reduced significantly, the expression level of SFTPC protein in the alveolar and interalveolar regions of lung tissue was reduced significantly, and the number of positive cells was low. Conclusions Combination of Scutellariae Radix-Forsythiae fructus(1 ∶ 1, 2 ∶ 3, 1 ∶ 2) has a significant protective effect on LPS-induced acute pneumonia.
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