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李美宁,马庆,弓韬,张引红,闫萍,翟翔,郭睿.小鼠 Spata3 蛋白结构和功能的生信分析及初鉴[J].中国实验动物学报,2022,30(6):767~776.
小鼠 Spata3 蛋白结构和功能的生信分析及初鉴
Bioinformatics analysis and primary identification of the structure and function of mouse Spata3 protein
投稿时间:2022-04-13  
DOI:10. 3969 / j.issn.1005-4847. 2022. 06. 005
中文关键词:  Spata3  精子发生  生物信息学  蛋白质修饰位点  蛋白质相互作用
英文关键词:Spata3  spermatogenesis  bioinformatics  protein modification site  protein-protein interaction
基金项目:
作者单位
李美宁 山西医科大学生物化学与分子生物学教研室,出生缺陷与细胞再生山西省重点实验室,太原 030001 
马庆 山西医科大学生物化学与分子生物学教研室,出生缺陷与细胞再生山西省重点实验室,太原 030001 
弓韬 山西医科大学生物化学与分子生物学教研室,出生缺陷与细胞再生山西省重点实验室,太原 030001 
张引红 山西 医科大学实验动物中心,实验动物与人类疾病动物模型山西省重点实验室,太原 030001 
闫萍 山西医科大学生物化学与分子生物学教研室,出生缺陷与细胞再生山西省重点实验室,太原 030001 
翟翔 山西医科大学生物化学与分子生物学教研室,出生缺陷与细胞再生山西省重点实验室,太原 030001 
郭睿 山西医科大学生物化学与分子生物学教研室,出生缺陷与细胞再生山西省重点实验室,太原 030001 
Author NameAffiliation
LI Meining Department of Biochemistry and Molecular Biology, Shanxi Medical University, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Taiyuan 030001, China 
MA Qing Department of Biochemistry and Molecular Biology, Shanxi Medical University, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Taiyuan 030001, China 
GONG Tao Department of Biochemistry and Molecular Biology, Shanxi Medical University, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Taiyuan 030001, China 
ZHANG Yinhong Department of Laboratory Animal Science, Shanxi Medical University, Shanxi Key Laboratory of Laboratory Animal and Animal Model of Human Diseases, Taiyuan 030001 
YAN Ping Department of Biochemistry and Molecular Biology, Shanxi Medical University, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Taiyuan 030001, China 
ZHAI Xiang Department of Biochemistry and Molecular Biology, Shanxi Medical University, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Taiyuan 030001, China 
GUO Rui Department of Biochemistry and Molecular Biology, Shanxi Medical University, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Taiyuan 030001, China 
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中文摘要:
       目的 对小鼠精子发生相关蛋白 3(spermatogenesis associated protein 3,Spata3)的序列进行分析,探 讨其结构和功能。 方法 利用 NCBI 数据库比对小鼠 Spata3 的同源性、ExPASy ProtParam 软件分析理化特征、 SOPMA 及 GOR4 预测空间构象、NetPhos3. 1 及 STRING 数据库分析蛋白修饰位点及蛋白间相互作用关系等信息、 免疫组化和免疫荧光染色检测其组织细胞定位。 结果 小鼠和人的 Spata3 基因 CDS 序列 64%相同,是碱性不稳定 亲水蛋白,无信号肽,属于非跨膜的胞内蛋白,主要定位于睾丸组织各级精子细胞核和胞质,以圆形精子细胞表达量最高。 小鼠 Spata3 含 1 个内在无序区结构域,30 个潜在的磷酸化位点,11 个潜在的 O-型糖基化位点,可能与 Spata46、Spert 等蛋白相互作用。 结论 小鼠 Spata3 是精子发生过程中的保守蛋白,可能调节精子发生变形过程。
英文摘要:
       Objective To analyze the sequence of mouse spermatogenesis associated protein 3 ( Spata3) and explore its structure and function. Methods The NCBI database was used to compare the homology of mouse Spata3. ExPASy ProtParam software was used to analyze its physical and chemical characteristics. SOPMA and GOR4 predicted the spatial conformation. NetPhos3. 1 and the STRING database were used to analyze protein modification sites and protein- protein interactions. Immunohistochemistry and immunofluorescence were used to observe the localization of Spata3 protein. Results Mouse Spata3 had 64% homology with humans in coding sequence. It was a basic unstable hydrophilic protein without a signal peptide. It was a non-transmembrane intracellular protein. It was mainly located in the nucleus and cytoplasm of sperm at all levels of testicular tissue, and its expression in round sperm cells was the highest. Spata3 contained an internal disordered domain, 30 potential phosphorylation sites, and 11 potential O-glycosylation sites, which may interact with proteins such as Spata46 and Spert. Conclusions Spata3 is a conserved protein in spermatogenesis and may regulate spermatogenesis.
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