Mastitis is one of the high incidence diseases in dairy farm,and the traditional therapies include using of antibiotics which usually have a high recurrent rate.The disease recurrence will reduce the milk production and cause worse result such as breast necrosis.So many cows are obsoleted because of mastitis and that cause a great economic loss to the dairy industry.Nursing mothers also suffering from mastitis and they cannot be treated with antibiotics because the medicine has a bad influence on the baby's nervous system.With all of the negative effects,antibiotics cannot be used in the mastitis treatment.Therefore it is extremely urgent to develop a new therapy for mastitis.Probiotics is a viable alternative which can be used in the prevention and treatment of mastitis because of many successful applications.Many previous examples in a variety of disease prevention and treatment using probiotics make this research very interesting.Digestive tract is the main niche where probiotics can play a crucial role in maintaining the host's health.It has been observed that digestive tract is one of the important microbial niche,which have a robust relationship with mastitis.It is the major issue of this research to use probiotic instead of antibiotics in the therapy of mastitis.The healthy intestinal microflora structure is crucial for the cows'health.Probiotics has a potential ability to cure mastitis in dairy cows.In addition it plays an essential role in maintaining the healthy state and mastitis prevention.