利用微卫星标记对四带无须鲃不同种群的遗传质量评价
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Genetic quality evaluation in different populations of tiger barb, Puntius tetrazona used microsatellite markers
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National Science and Technology Support Programme

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    摘要:

    目的 分析四带无须鲃封闭群及近交群的遗传质量。 方法 筛选多态性丰富的四带无须鲃微卫星序列,通过构建两个多重PCR反应体系再利用毛细管电泳技术进行分型,开展群体遗传多样性分析。 结果 野生群、WT封闭群、BT封闭群及近交群的平均等位基因数分别为6.5833、3.1667、3.0833和3.1818,平均多态信息含量分别为0.5969、0.3748、0.4159和0.4241。群体遗传质量检测分析表明:4个群体之间遗传分化结果和近交群由野生群、封闭群逐渐筛选获得的过程相符。结论 筛选的微卫星标记可用于四带无须鲃不同群体的遗传质量分析,为其遗传质量控制及监测提供方法基础。

    Abstract:

    Objective To analyze and evaluate the genetic quality of closed populations and inbred population of tiger barb, Puntius tetrazona. Methods The microsatellite sequences with abundant polymorphism in tiger barb were screened, and two multiple PCR reaction systems were established. Then four populations were analyzed by capillary electrophoresis to identified the genotype and evaluated the genetic diversity. Results The average number of alleles in wild group, WT closed group, BT closed group, and inbred group were 6.5833, 3.1667, 3.0833, and 3.1818. The average polymorphic information content were 0.5969, 0.3748, 0.4159 and 0.4241, respectively. The analysis of population genetic quality showed that the results of genetic differentiation between the four groups were consistent with the process of the inbred population bred from the wild and closed group gradually. Conclusions These microsatellite markers can be used to analyze the genetic quality of different populations of tiger barb, providing a methodological basis for their genetic quality control and monitoring.

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陈晓虹,张新铖,刘春,王芳,李凯彬.利用微卫星标记对四带无须鲃不同种群的遗传质量评价[J].中国实验动物学报,,().

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  • 收稿日期:2018-06-04
  • 最后修改日期:2018-06-15
  • 录用日期:2018-06-19
  • 在线发布日期: 2018-11-12
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