目的 寻找诊断和治疗肠易激综合征（IBS）的生物学指标，探索戊己丸治疗IBS的作用机制。方法 采用乙酸加束缚应激法建立炎症后肠易激综合征（PI-IBS）动物模型；采用大鼠结肠运动指数（MI）、2 h内排出的粪粒数以及玻璃小球排出时间评价大鼠结肠的运动能力；观察PI-IBS模型大鼠的形成以及戊己丸对其的治疗作用；还采用ELISA法检测PI-IBS大鼠脑和结肠组织中的降钙素基因相关肽（CGRP）、胃动素（MTL）、神经肽Y(NPY)、P物质（SP）、生长抑素（SS）、血管活性肠肽（VIP）、胆囊收缩素（CCK）水平。结果 本方法可成功建立PI-IBS大鼠。模型大鼠体重降低；摄食量减少；排便量增多；出现稀便和无定形软便；自主运动量减少；结肠MI显著增加（P<0.05）；大鼠排出的粪粒数显著增加（P<0.05）；玻璃小球排出时间显著缩短（P<0.05）。戊己丸治疗7 d后能够明显改善各种症状。与正常对照相比，PI-IBS大鼠脑组织中的CGRP、SS和VIP水平显著增加（P<0.05），NPY浓度显著降低（P<0.05）；而戊己丸给药可以显著降低CGRP、SS和VIP浓度水平（P<0.05），显著升高NPY浓度水平（P<0.05）。与正常对照相比，PI-IBS大鼠结肠组织中的CCK、NPY、MTL、SS和VIP均显著降低（P<0.05）；戊己丸给药能够显著升高CCK和VIP水平（P<0.05）。结论 戊己丸可通过调节IBS大鼠脑和结肠组织内多种脑肠肽的水平来治疗IBS，这些可被调节的异常变化的脑肠肽可能成为潜在的诊断和治疗IBS的生物学指标。
Objective To explore the biological indicator of diagnosis and treatment of irritable bowel syndrome (IBS), to explore the mechanism of action of Wuji Pill on Irritable Bowel Syndrome (IBS). Methods (1) Post Inflammatory Irritable Bowel Syndrome (PI-IBS) animal model was established by acetic acid plus restraint stress method. (2) The colonic motor ability of rats was evaluated by colon motility index (MI), the number of fecal particles discharged within 2 h, and the time of glass pellet discharged. (3) The formation of PI-IBS model rats and the therapeutic effect of WJW was observed. (4) The levels of calcitonin gene-related peptide (CGRP), motilin (MTL), neuropeptide Y (NPY), substance P (SP), somatostatin (SS), vasoactive intestinal peptide (VIP), and cholecystokinin (CCK) in the brain and colon tissues of PI-IBS rats were measured by ELISA. Results (1) successfully established the rat PI-IBS model. Compared with the normal group, the weight of the model rats decreased; the food intake decreased; the amount of defecation increased; loose stools and amorphous soft stools appeared; autonomic exercise decreased; colon MI increased significantly (P <0.05); the number of fecal particles discharged increased significantly (P <0.05); the glass pellet discharge time was significantly shortened (P <0.05). (2) WJW treatment 7d can significantly improve a variety of symptoms. Compared with the normal control, the levels of CGRP, SS and VIP in the brain tissue of PI-IBS rats were significantly increased (P <0.05), and the NPY concentration was significantly decreased (P <0.05). However, the treatment of WJW can significantly reduce CGRP, SS and VIP levels (P<0.05); and significantly increased the NPY concentration level (P<0.05). (3) Compared with the normal control, the level of CCK, NPY, MTL, SS and VIP in colonic tissue of PI-IBS rats were significantly decreased (P<0.05), while WJW can significantly increase CCK and VIP levels. Conclusion WJW can treat IBS by regulating the levels of various brain-gut peptides in the brain and colon tissue of IBS rats. These adjustable and anomalous brain-gut peptide may be a potential biomarker for the diagnosis and treatment of IBS.