黑线仓鼠及其白化突变系酪氨酸酶基因的克隆与序列分析
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赵彦斌(1984-),男,在读硕士研究生;研究方向:实验动物学。

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国家自然科学基金资助项目(项目批准号 30870347)。


Cloning and sequence analysis of tyrosinase gene in Cricetulus barabensis and albino mutant Cricetulus barabensis
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    摘要:

    目的对黑线仓鼠及其白化突变系酪氨酸酶基因进行比较研究,揭示黑线仓鼠白化突变系白化性状产生的分子机理。方法根据小鼠与大鼠的酪氨酸酶基因保守区设计3对引物,利用RT-PCR方法 , 从黑线仓鼠及其白化系皮肤总 RNA中扩增得到酪氨酸酶的cDNA基因,并对其二者进行克隆测序。结果成功获得了黑线仓鼠及其白化突变系的酪氨酸酶基因,对二者的序列比较分析结果表明,二者的编码区没有差异。 结论黑线仓鼠白化突变系白化性状产生的原因与已知小鼠的白化性状产生原因不同,并不是由酪氨酸酶基因编码区突变造成的,其白化性状产生的机理有待进一步的研究。

    Abstract:

    ObjectiveIn order to reveal the molecular mechanism of the albino mutant Cricetulus barabensis, the tyrosinase gene of Cricetulus barabensis and the albino mutant Cricetulus barabensis was cloned, sequenced, and comparatively analyzed. MethodsThe Tyrosinase cDNA was amplified from the total RNA of the skin of Cricetulus barabensis and its albino mutant Cricetulus barabensis by reverse transcriptase (RT-PCR). According to the conservative region of tyrosinase gene of mice and rats, three pairs of primers were designed to link three fragments to a long epitope by overlap extension. Then the three fragments were amplified and sequenced and finally spliced into the complete sequence. The mutation site was identified through DNA matching software.ResultsThe tyrosinase genes of Cricetulus barabensis and the albino mutant Cricetulus barabensis were cloned and the mutation site was measured by DNA sequence analysis software. The results showed that they have the same coding region and there is no mutation.ConclusionsThe reason of the albino trait of the albino mutant Cricetulus barabensis is different from that of albino trait known in mice, not by the mutation of tyrosinase gene coding region, and reqires further investigation.

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赵彦斌,孙兆增,叶华虎,王冬平,胡仲明,曾林.黑线仓鼠及其白化突变系酪氨酸酶基因的克隆与序列分析[J].中国实验动物学报,2012,(1):5~9.

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